Record for CD0000031000 (Elastase)

Sample

PCDDBID CD0000031000 ?
Protein Name Elastase ?
Alternative Protein Names Chymotrypsin-like elastase family member 1, Pancreatic Elastase ?
Source Organism Sus scrofa ?
Protein Supplier No data provided ?
Expression System or natural source natural source ?
Expressed As Wild-type ?
Mutation Details No data provided ?
Expression tags (if any) No data provided ?
Ligands Present and Concentration or ratio No data provided ?
Macromolecular Partner(s) and Concentration or ratio No data provided ?
Deposition Date 2009-11-26 ?

Spectra

PCDDBID CD0000031000 ?
Raw Sample Spectra file   chart ?
Dichroism Units of Raw Sample Data Millidegrees ?
HT / High Voltage / Dynode Spectra file   chart ?
Raw Baseline Spectra file   chart ?
Dichroism Units of Raw Baseline Data Millidegrees ?
Average Sample file   chart ?
Dichroism Units of Average Sample Data Millidegrees ?
Averaged Baseline file   chart ?
Dichroism Units of Averaged Baseline Data Millidegrees ?
Net Smoothed Spectrum file   chart ?
Dichroism Units of Net Smoothed Data Millidegrees ?
Final Processed Spectrum file   chart ?
Dichroism Units of Processed Data Delta Epsilon ?

Experiment

PCDDBID CD0000031000 ?
CD or SRCD SRCD ?
Protein Concentration (mg/ml) 8.15 ?
Concentration Quantification Method QAA ?
Protein Purity (%) No data provided ?
Purity Quantification Method No data provided ?
Buffer Contents and Concentrations water ?
Baseline Contents water ?
Experimental Temperature (C) 4 ?
Instrument or beamline Daresbury 12.1 ?
Detector Angle (Scattering Angle) No data provided ?
Sample Cell Pathlength (cm) 0.00063 ?
Cell Pathlength Calibration Method Interferometry ?
Sample Cell Type Cylindrical-Demountable ?
Sample Cell Composition CaF2 ?
Sample Chamber Atmosphere Nitrogen ?
Number of repeat scans 3 ?
Continuous or Stepped scan No data provided ?
Maximum (highest) wavelength, nm 277 ?
Minimum (lowest) wavelength, nm 175 ?
Criteria for low wavelength cutoff HT value ?
Wavelength interval, nm 1 ?
Dwell or Averaging time, seconds 1 ?
Experimental Collection date 2004-11-24 ?
Local Spectrum Identifier r12664 ?

Calibration

PCDDBID CD0000031000 ?
CSA or ACS CSA ?
CSA/ACS Standard Spectrum file   chart ?
Dichroism Units for CSA Standard Millidegrees (theta) ?
Final Spectrum Calibrated No data provided ?
CSA/ACS Standard Concentration (mg/ml) 11.4 ?
CSA/ACS Pathlength (mm) 0.1 ?
CSA/ACS Zeroed at 235-245 ?
CSA/ACS Date Measured 2004-11-24 ?
CSA/ACS Ratio (192.5nm and 290.0 nm) 1.96 ?
CD signal at 290nm (mdeg) 37.4 ?
CSA/ACS Experiment temperature, C 25 ?

Data Process

PCDDBID CD0000031000 ?
Molecular Weight 25900 ?
Number of Residues 240 ?
Mean Residue Weight 108.4 ?
Data Processing Software Name CDTool ?
Data Processing Software Version 1.4 ?
Number of Smoothing Points 5 ?
Wavelength Range for Zeroing 263-270 ?

Sec. Struct.

PCDDBID CD0000031000 ?
Secondary Structure Calculated from PDB File ?
DSSP value: alpha helix 0.054 ?
DSSP value: 3-10 helix 0.042 ?
DSSP value: pi helix 0.000 ?
DSSP value: beta strand 0.304 ?
DSSP value: beta bridge 0.017 ?
DSSP value: bonded turn 0.138 ?
DSSP value: bend 0.054 ?
DSSP value: loop or irregular 0.392 ?

Info.

PCDDBID CD0000031000 ?
PDB ID 3est: RSCBpdb PDBj PDBe ?
UniProt ID P00772 ?
Enzyme Classification (EC) 3.4.21.36 ?
Medline Entry 16787970 (pubmed) ?
Cath Classification 2.40.10.10 ?
Sequence VVGGTEAQRN SWPSQISLQY RSGSSWAHTC GGTLIRQNWV MTAAHCVDRE LTFRVVVGEH NLNQNDGTEQ YVGVQKIVVH PYWNTDDVAA GYDIALLRLA QSVTLNSYVQ LGVLPRAGTI LANNSPCYIT GWGLTRTNGQ LAQTLQQAYL PTVDYAICSS SSYWGSTVKN SMVCAGGDGV RSGCQGDSGG PLHCLVNGQY AVHGVTSFVS RLGCNVTRKP TVFTRVSAYI SWINNVIASN ?
Type of protein soluble globular ?
Keyword/phrase A SP175 ?
Keyword/phrase B single domain type ?
Keyword/phrase C beta ?
Keyword/phrase D No data provided ?
Keyword/phrase E No data provided ?
Keyword/phrase F No data provided ?
Keyword/phrase G No data provided ?
Keyword/phrase H No data provided ?
Keyword/phrase I No data provided ?
Keyword/phrase J No data provided ?
Publication Authors Lees, J.G., Wien, F., Miles, A.J. & Wallace, B.A., ?
Publication Year 2006 ?
Publication Journal Bioinformatics ?
Publication Title A reference database for circular dichroism spectroscopy covering fold and secondary structure space ?
Publication Volume 22 ?
Publication Pages 1955-1962 ?
Citation Publication file ?

Depositor

PCDDBID CD0000031000 ?
Depositor Name Andrew Miles ?
Department/School name Crystallography ?
University/Institution/Corporation Birkbeck ?
Depositor Country United Kingdom ?
Name of Principal Investigator (if not depositor) B.A. Wallace ?

Validation

PCDDBID CD0000031000 Help icon, hover over for help text.
Maximum Delta Epsilon PASS Help icon, hover over for help text.
Minimal Level of Maximum Delta Epsilon PASS Help icon, hover over for help text.
Signal to noise ratio PASS Help icon, hover over for help text.
Deviations from zero PASS Help icon, hover over for help text.
High Tension Voltage readings PASS Help icon, hover over for help text.
Wavelength range covered (low end / high end) PASS PASS Help icon, hover over for help text.
Interval resolution PASS Help icon, hover over for help text.
Deviation between 260-270nm PASS Help icon, hover over for help text.
Mean Residue Weight value PASS Help icon, hover over for help text.
Flat topped peaks PASS Help icon, hover over for help text.
CSA or ACS standard used PASS Help icon, hover over for help text.
Width of peaks at half height PASS Help icon, hover over for help text.
Peak and trough locations PASS Help icon, hover over for help text.
Spectral Feature Width PASS Help icon, hover over for help text.
CSA / ACS peak ratio PASS Help icon, hover over for help text.
Calibration data at 290nm PASS Help icon, hover over for help text.
Sum of DSSP fractions PASS Help icon, hover over for help text.
Overall validation level is: PASS
Validation report compiled by Validichro v1.0, 2009-12-15, 13:00.
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