HNF-1A DBD

Citation: No citation information currently associated with this entry.

Citation: The PCDDB (protein circular dichroism data bank): A bioinformatics resource for protein characterisations and methods development.
Ramalli SG, Miles AJ, Janes RW, Wallace BA., J Mol Biol (2022)

CSA/ACS Standard Spectrum	Millidegrees_(theta)		File not provided
Raw Sample Spectra	Millidegrees_(theta)	Download	View Plot
Raw Sample Spectra 2	Millidegrees_(theta)	Download	View Plot
Raw Sample Spectra 3	Millidegrees_(theta)	Download	View Plot
Raw Baseline Spectra	Millidegrees_(theta)	Download	View Plot
Raw Baseline Spectra 2	Millidegrees_(theta)	Download	View Plot
Raw Baseline Spectra 3	Millidegrees_(theta)	Download	View Plot
Average Sample		Download	File not provided
Averaged Baseline		Download	File not provided
HT / High Voltage / Dynode Spectra	HT/Dynode Units	Download	File not provided
Net Smoothed Spectrum		Download	File not provided
Final Processed Spectrum	Delta_Epsilon	Download	View Plot

Validation report compiled by Validichro v1.4.0.1, 2023-09-08 11:59:31. - PASS (incomplete)

At a glance

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Sample

Protein Name	HNF-1A DBD	?
Alternative Protein Names	No data provided	?
Source Organism	Homo sapiens	?
Protein Supplier	Thomas Arnesen lab, University of Bergen, Department of Biomedicine, Bergen, Norway	?
Expression System or natural source	E. coli	?
Expressed As	Other-Specify	?
Mutation Details	residues 83 - 279	?
Expression tags (if any)	N, G	?
Ligands Present and Concentration or ratio	No data provided	?
Macromolecular Partner(s) and Concentration or ratio	No data provided	?
Deposition Date	2023-08-28	?

Experiment

CD or SRCD	SRCD	?
Protein Concentration (mg/ml)	0.5	?
Concentration Quantification Method	Nanodrop	?
Protein Purity (%)	No data provided	?
Purity Quantification Method	No data provided	?
Buffer Contents and Concentrations	20 mM sodium phosphate pH 7.8, 150 mM NaF, 1 mM TCEP	?
Baseline Contents	20 mM sodium phosphate pH 7.8, 150 mM NaF, 1 mM TCEP	?
Experimental Temperature (C)	10	?
Instrument or beamline	AU-CD beamline, ASTRID2 synchrotron (Aarhus, Denmark)	?
Detector Angle (Scattering Angle)	No data provided	?
Sample Cell Pathlength (cm)	0.01	?
Cell Pathlength Calibration Method	Manufacturers Spec.	?
Sample Cell Type	Rectangular	?
Sample Cell Composition	Quartz	?
Sample Chamber Atmosphere	Nitrogen	?
Number of repeat scans	3	?
Continuous or Stepped scan	Stepped	?
Maximum (highest) wavelength, nm	280	?
Minimum (lowest) wavelength, nm	170	?
Criteria for low wavelength cutoff	HT value	?
Wavelength interval, nm	1	?
Dwell or Averaging time, seconds	No data provided	?
Experimental Collection date	2021-12-13	?
Local Spectrum Identifier	No data provided	?

Calibration

CSA or ACS	CSA	?
Dichroism Units for CSA Standard	Millidegrees_(theta)	?
Final Spectrum Calibrated	NO	?
CSA/ACS Standard Concentration (mg/ml)	No data provided	?
CSA/ACS Pathlength (mm)	No data provided	?
CSA/ACS Zeroed at	No data provided	?
CSA/ACS Date Measured	No data provided	?
CSA/ACS Ratio (192.5nm and 290.0 nm)	No data provided	?
CD signal at 290nm (mdeg)	No data provided	?
CSA/ACS Experiment temperature, C	No data provided	?

Data process.

Molecular Weight	22840	?
Number of Residues	198	?
Mean Residue Weight	115.4	?
Data Processing Software Name	Other-Specify, Excel	?
Data Processing Software Version	No data provided	?
Wavelength Range for Zeroing	No data provided	?
Number of Smoothing Points	No data provided	?

Sec. struct.

Secondary Structure Calculated from	No data provided	?
DSSP value: alpha helix	No data provided	?
DSSP value: 3-10 helix	No data provided	?
DSSP value: pi helix	No data provided	?
DSSP value: beta strand	No data provided	?
DSSP value: beta bridge	No data provided	?
DSSP value: bonded turn	No data provided	?
DSSP value: bend	No data provided	?
DSSP value: loop or irregular	No data provided	?

Tertiary

PDB ID	No data provided	?
UniProt ID	P20823	?
Enzyme Classification (EC)	No data provided	?
Medline Entry	No data provided	?
Cath Classification	No data provided	?
Sequence	GPPILKELEN LSPEEAAHQK AVVETLLQED PWRVAKMVKS YLQQHNIPQR EVVDTTGLNQ SHLSQHLNKG TPMKTQKRAA LYTWYVRKQR EVAQQFTHAG QGGLIEEPTG DELPTKKGRR NRFKWGPASQ QILFQAYERQ KNPSKEERET LVEECNRAEC IQRGVSPSQA QGLGSNLVTE VRVYNWFANR RKEEAFRH	?
Type of protein	soluble_globular	?
Keyword/phrase A	transcription factor	?
Keyword/phrase B	No data provided	?
Keyword/phrase C	No data provided	?
Keyword/phrase D	No data provided	?
Keyword/phrase E	No data provided	?
Keyword/phrase F	No data provided	?
Keyword/phrase G	No data provided	?
Keyword/phrase H	No data provided	?
Keyword/phrase I	No data provided	?
Keyword/phrase J	No data provided	?
Publication Authors	No data provided	?
Publication Year	No data provided	?
Publication Journal	No data provided	?
Publication Title	No data provided	?
Publication Volume	No data provided	?
Publication Pages	No data provided	?

Depositor

Depositor Name	Laura Kind	?
Department/School name	Department of Biomedicine	?
University/Institution/Corporation	University of Bergen	?
Depositor Country	Norway	?
Name of Principal Investigator (if not depositor)	Prof. Petri Kursula	?

Validation report compiled by Validichro v1.4.0.1, 2023-09-08 11:59:31. - PASS (incomplete)

Depositors Notes:

Missing Wavelengths	PASS	?
Maximum Delta Epsilon	INSUFFICIENT DATA	?
Minimal Level of Maximum Delta Epsilon	INSUFFICIENT DATA	?
Peak Locations	INSUFFICIENT DATA	?
Feature Width	PASS	?
Experimental Temperature	PASS	?
UniProt sequence		?
Molecular Weight	PASS	?
Number of Residues	PASS	?
Mean Residue Weight value	PASS	?
Concentration and Pathlength	PASS	?
CSA / ACS peak ratio	INSUFFICIENT DATA	?
CSA / ACS Temperature	INSUFFICIENT DATA	?
Peak Shift test	PASS	?
Standard Deviation	PASS	?
Noise: 260-270nm	INSUFFICIENT DATA	?
Flat topped peaks	PASS	?
Wavelength range	INSUFFICIENT DATA	?
Interval resolution	PASS	?
High Tension Voltage 240-260nm	INSUFFICIENT DATA	?
Projection Test	INSUFFICIENT DATA	?
Standard Deviation At Peak	PASS	?
Depositor Note		?