View & Download CD data for CD0003893000
Alpha-2-macroglobulin (c3 and pzp-like alpha-2-macroglobulin domain-containing protein 5)
Citation: No citation information currently associated with this entry.
Citation: The PCDDB (protein circular dichroism data bank): A bioinformatics resource for protein characterisations and methods development.
Ramalli SG, Miles AJ, Janes RW, Wallace BA., J Mol Biol (2022)
| CSA/ACS Standard Spectrum | ---- Please select ---- | Download |
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| Raw Sample Spectra | Millidegrees (theta) | Download |
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| Raw Sample Spectra 2 | Millidegrees (theta) | Download |
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| Raw Sample Spectra 3 | Millidegrees (theta) | Download |
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| Raw Sample Spectra 4 | Millidegrees (theta) | Download |
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| Raw Sample Spectra 5 | Millidegrees (theta) | Download |
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| Raw Baseline Spectra | Millidegrees (theta) | Download |
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| Raw Baseline Spectra 2 | Millidegrees (theta) | Download |
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| Raw Baseline Spectra 3 | Millidegrees (theta) | Download |
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| Raw Baseline Spectra 4 | Millidegrees (theta) | Download |
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| Raw Baseline Spectra 5 | Millidegrees (theta) | Download |
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| Average Sample | Millidegrees (theta) | Download |
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| Averaged Baseline | Millidegrees (theta) | Download |
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| HT / High Voltage / Dynode Spectra | HT/Dynode Units | Download |
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| HT / High Voltage / Dynode Spectra 2 | HT/Dynode Units | Download |
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| HT / High Voltage / Dynode Spectra 3 | HT/Dynode Units | Download |
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| HT / High Voltage / Dynode Spectra 4 | HT/Dynode Units | Download |
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| HT / High Voltage / Dynode Spectra 5 | HT/Dynode Units | Download |
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| Net Smoothed Spectrum | Millidegrees (theta) | Download |
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| Final Processed Spectrum | Delta Epsilon | Download |
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Validation report compiled by Validichro v1.4.0, 2015-04-03, 10:04am. - PASS
At a glance
| Downloads | 2510 |
| Depositor | Frank Wien |
| Uniprot |
P01023 |
| Alpha Fold | |
| PDB |
4ACQ |
| EC | |
| CATH Class | |
| Protein Type | soluble globular |
Sample
| Protein Name | Alpha-2-macroglobulin | ? |
| Alternative Protein Names | C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5 | ? |
| Source Organism | Homo sapiens | ? |
| Protein Supplier | Jzsef Dob, Institute of Enzymology | ? |
| Expression System or natural source | E. coli | ? |
| Expressed As | Wild-type | ? |
| Mutation Details | No data provided | ? |
| Expression tags (if any) | No data provided | ? |
| Ligands Present and Concentration or ratio | No data provided | ? |
| Macromolecular Partner(s) and Concentration or ratio | No data provided | ? |
| Deposition Date | 2015-04-03 | ? |
Experiment
| CD or SRCD | SRCD | ? |
| Protein Concentration (mg/ml) | 5.55 | ? |
| Concentration Quantification Method | QAA | ? |
| Protein Purity (%) | >95% | ? |
| Purity Quantification Method | SDS gels | ? |
| Buffer Contents and Concentrations | 20mMTris-Cl, 0,1mM EDTA,150mM NaCl, pH7.5 | ? |
| Baseline Contents | dialysate | ? |
| Experimental Temperature (C) | 25 | ? |
| Instrument or beamline | Soleil DISCO | ? |
| Detector Angle (Scattering Angle) | 60 | ? |
| Sample Cell Pathlength (cm) | 0.0012 | ? |
| Cell Pathlength Calibration Method | Interferometry | ? |
| Sample Cell Type | Cylindrical-Demountable | ? |
| Sample Cell Composition | CaF2 | ? |
| Sample Chamber Atmosphere | Nitrogen | ? |
| Number of repeat scans | 5 | ? |
| Continuous or Stepped scan | Stepped | ? |
| Maximum (highest) wavelength, nm | 275 | ? |
| Minimum (lowest) wavelength, nm | 180 | ? |
| Criteria for low wavelength cutoff | HT value | ? |
| Wavelength interval, nm | 1 | ? |
| Dwell or Averaging time, seconds | 1.2 | ? |
| Experimental Collection date | 2012-09-08 | ? |
| Local Spectrum Identifier | paprika4 | ? |
Calibration
| CSA or ACS | CSA | ? |
| Dichroism Units for CSA Standard | No data provided | ? |
| Final Spectrum Calibrated | YES | ? |
| CSA/ACS Standard Concentration (mg/ml) | 6.3 | ? |
| CSA/ACS Pathlength (mm) | 0.1 | ? |
| CSA/ACS Zeroed at | 230-245 | ? |
| CSA/ACS Date Measured | 2012-09-08 | ? |
| CSA/ACS Ratio (192.5nm and 290.0 nm) | 1.95 | ? |
| CD signal at 290nm (mdeg) | 19.72 | ? |
| CSA/ACS Experiment temperature, C | 25 | ? |
Sec. struct.
Tertiary
| PDB ID | 4ACQ | ? |
| UniProt ID | P01023 | ? |
| Enzyme Classification (EC) | No data provided | ? |
| Medline Entry | No data provided | ? |
| Cath Classification | No data provided | ? |
| Sequence | SVSGKPQYMV LVPSLLHTET TEKGCVLLSY LNETVTVSAS LESVRGNRSL FTDLEAENDV LHCVAFAVPK SSSNEEVMFL TVQVKGPTQE FKKRTTVMVK NEDSLVFVQT DKSIYKPGQT VKFRVVSMDE NFHPLNELIP LVYIQDPKGN RIAQWQSFQL EGGLKQFSFP LSSEPFQGSY KVVVQKKSGG RTEHPFTVEE FVLPKFEVQV TVPKIITILE EEMNVSVCGL YTYGKPVPGH VTVSICRKYS DASDCHGEDS QAFCEKFSGQ LNSHGCFYQQ VKTKVFQLKR KEYEMKLHTE AQIQEEGTVV ELTGRQSSEI TRTITKLSFV KVDSHFRQGI PFFGQVRLVD GKGVPIPNKV IFIRGNEANY YSNATTDEHG LVQFSINTTN VMGTSLTVRV NYKDRSPCYG YQWVSEEHEE AHHTAYLVFS PSKSFVHLEP MSHELPCGHT QTVQAHYILN GGTLLGLKKL SFYYLIMAKG GIVRTGTHGL LVKQEDMKGH FSISIPVKSD IAPVARLLIY AVLPTGDVIG DSAKYDVENC LANKVDLSFS PSQSLPASHA HLRVTAAPQS VCALRAVDQS VLLMKPDAEL SASSVYNLLP EKDLTGFPGP LNDQDNEDCI NRHNVYINGI TYTPVSSTNE KDMYSFLEDM GLKAFTNSKI RKPKMCPQLQ QYEMHGPEGL RVGFYESDVM GRGHARLVHV EEPHTETVRK YFPETWIWDL VVVNSAGVAE VGVTVPDTIT EWKAGAFCLS EDAGLGISST ASLRAFQPFF VELTMPYSVI RGEAFTLKAT VLNYLPKCIR VSVQLEASPA FLAVPVEKEQ APHCICANGR QTVSWAVTPK SLGNVNFTVS AEALESQELC GTEVPSVPEH GRKDTVIKPL LVEPEGLEKE TTFNSLLCPS GGEVSEELSL KLPPNVVEES ARASVSVLGD ILGSAMQNTQ NLLQMPYGCG EQNMVLFAPN IYVLDYLNET QQLTPEIKSK AIGYLNTGYQ RQLNYKHYDG SYSTFGERYG RNQGNTWLTA FVLKTFAQAR AYIFIDEAHI TQALIWLSQR QKDNGCFRSS GSLLNNAIKG GVEDEVTLSA YITIALLEIP LTVTHPVVRN ALFCLESAWK TAQEGDHGSH VYTKALLAYA FALAGNQDKR KEVLKSLNEE AVKKDNSVHW ERPQKPKAPV GHFYEPQAPS AEVEMTSYVL LAYLTAQPAP TSEDLTSATN IVKWITKQQN AQGGFSSTQD TVVALHALSK YGAATFTRTG KAAQVTIQSS GTFSSKFQVD NNNRLLLQQV SLPELPGEYS MKVTGEGCVY LQTSLKYNIL PEKEEFPFAL GVQTLPQTCD EPKAHTSFQI SLSVSYTGSR SASNMAIVDV KMVSGFIPLK PTVKMLERSN HVSRTEVSSN HVLIYLDKVS NQTLSLFFTV LQDVPVRDLK PAIVKVYDYY ETDEFAIAEY NAPCSKDLGN A | ? |
| Type of protein | soluble globular | ? |
| Keyword/phrase A | Hydrolase inhibitor | ? |
| Keyword/phrase B | No data provided | ? |
| Keyword/phrase C | No data provided | ? |
| Keyword/phrase D | No data provided | ? |
| Keyword/phrase E | No data provided | ? |
| Keyword/phrase F | No data provided | ? |
| Keyword/phrase G | No data provided | ? |
| Keyword/phrase H | No data provided | ? |
| Keyword/phrase I | No data provided | ? |
| Keyword/phrase J | No data provided | ? |
| Publication Authors | No data provided | ? |
| Publication Year | No data provided | ? |
| Publication Journal | No data provided | ? |
| Publication Title | No data provided | ? |
| Publication Volume | No data provided | ? |
| Publication Pages | No data provided | ? |
Validation report compiled by Validichro v1.4.0, 2015-04-03, 10:04am. - PASS
Depositors Notes: Validation completed fine spectra
| Missing Wavelengths | PASS | ? |
| Maximum Delta Epsilon | PASS | ? |
| Minimal Level of Maximum Delta Epsilon | PASS | ? |
| Peak Locations | PASS | ? |
| Feature Width | PASS | ? |
| Experimental Temperature | PASS | ? |
| UniProt sequence | PASS | ? |
| Molecular Weight | PASS | ? |
| Number of Residues | PASS | ? |
| Mean Residue Weight value | PASS | ? |
| Concentration and Pathlength | PASS | ? |
| CSA / ACS peak ratio | PASS | ? |
| CSA / ACS Temperature | PASS | ? |
| Peak Shift test | PASS | ? |
| Standard Deviation | PASS | ? |
| Noise: 260-270nm | PASS | ? |
| Flat topped peaks | PASS | ? |
| Wavelength range | PASS | ? |
| Interval resolution | PASS | ? |
| High Tension Voltage 240-260nm | PASS | ? |
| Projection Test | PASS | ? |
| Standard Deviation At Peak | PASS | ? |
| Depositor Note | Validation completed fine spectra | ? |