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Record for CD0004676000 (Calexcitin)

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Sample

PCDDBIDCD0004676000Help icon, hover over for help text.
Protein NameCalexcitinHelp icon, hover over for help text.
Alternative Protein NamesNo data providedHelp icon, hover over for help text.
Source OrganismL. pealeiHelp icon, hover over for help text.
Protein SupplierJ.B. Cooper, Royal Free Hospital, London, UKHelp icon, hover over for help text.
Expression System or natural sourceE. coliHelp icon, hover over for help text.
Expressed AsWild-typeHelp icon, hover over for help text.
Mutation DetailsNo data providedHelp icon, hover over for help text.
Expression tags (if any)noneHelp icon, hover over for help text.
Ligands Present and Concentration or rationoneHelp icon, hover over for help text.
Macromolecular Partner(s) and Concentration or rationoneHelp icon, hover over for help text.
Deposition Date2015-03-16Help icon, hover over for help text.

Spectra

PCDDBIDCD0004676000Help icon, hover over for help text.
Raw Sample Spectra file   chartHelp icon, hover over for help text.
Dichroism Units of Raw Sample DataMillidegrees (theta)Help icon, hover over for help text.
HT / High Voltage / Dynode Spectra file   chartHelp icon, hover over for help text.
Raw Baseline Spectra file   chartHelp icon, hover over for help text.
Dichroism Units of Raw Baseline DataMillidegrees (theta)Help icon, hover over for help text.
Average Sample file   chartHelp icon, hover over for help text.
Dichroism Units of Average Sample DataMillidegrees (theta)Help icon, hover over for help text.
Averaged Baseline file   chartHelp icon, hover over for help text.
Dichroism Units of Averaged Baseline DataMillidegrees (theta)Help icon, hover over for help text.
Net Smoothed Spectrum file   chartHelp icon, hover over for help text.
Dichroism Units of Net Smoothed DataMillidegrees (theta)Help icon, hover over for help text.
Final Processed Spectrum file   chartHelp icon, hover over for help text.
Dichroism Units of Processed DataDelta EpsilonHelp icon, hover over for help text.

Experiment

PCDDBIDCD0004676000Help icon, hover over for help text.
CD or SRCDCDHelp icon, hover over for help text.
Protein Concentration (mg/ml)1.77Help icon, hover over for help text.
Concentration Quantification MethodNanodropHelp icon, hover over for help text.
Protein Purity (%)No data providedHelp icon, hover over for help text.
Purity Quantification MethodcrystallisationHelp icon, hover over for help text.
Buffer Contents and Concentrations50mM phosphate pH 7.5, 0.1% mercaptoethanol, 1mM CaClHelp icon, hover over for help text.
Baseline Contents50mM phosphate pH 7.5, 0.1% mercaptoethanol, 1mM CaClHelp icon, hover over for help text.
Experimental Temperature (C)25Help icon, hover over for help text.
Instrument or beamlineAvivHelp icon, hover over for help text.
Detector Angle (Scattering Angle)No data providedHelp icon, hover over for help text.
Sample Cell Pathlength (cm)0.0015Help icon, hover over for help text.
Cell Pathlength Calibration MethodInterferometryHelp icon, hover over for help text.
Sample Cell TypeCylindrical-DemountableHelp icon, hover over for help text.
Sample Cell CompositionSuprasilHelp icon, hover over for help text.
Sample Chamber AtmosphereNitrogenHelp icon, hover over for help text.
Number of repeat scans3Help icon, hover over for help text.
Continuous or Stepped scanSteppedHelp icon, hover over for help text.
Maximum (highest) wavelength, nm280Help icon, hover over for help text.
Minimum (lowest) wavelength, nm180Help icon, hover over for help text.
Criteria for low wavelength cutoffHT valueHelp icon, hover over for help text.
Wavelength interval, nm1Help icon, hover over for help text.
Dwell or Averaging time, seconds1Help icon, hover over for help text.
Experimental Collection date2012-12-23Help icon, hover over for help text.
Local Spectrum Identifieralex03Help icon, hover over for help text.

Calibration

PCDDBIDCD0004676000Help icon, hover over for help text.
CSA or ACSCSAHelp icon, hover over for help text.
CSA/ACS Standard Spectrum file   chartHelp icon, hover over for help text.
Dichroism Units for CSA StandardMillidegrees \(theta\)Help icon, hover over for help text.
Final Spectrum CalibratedYESHelp icon, hover over for help text.
CSA/ACS Standard Concentration (mg/ml)8.4Help icon, hover over for help text.
CSA/ACS Pathlength (mm)0.1Help icon, hover over for help text.
CSA/ACS Zeroed at235-245Help icon, hover over for help text.
CSA/ACS Date Measured2012-02-22Help icon, hover over for help text.
CSA/ACS Ratio (192.5nm and 290.0 nm)2.14Help icon, hover over for help text.
CD signal at 290nm (mdeg)30Help icon, hover over for help text.
CSA/ACS Experiment temperature, C25Help icon, hover over for help text.

Data Process

PCDDBIDCD0004676000Help icon, hover over for help text.
Molecular Weight22271Help icon, hover over for help text.
Number of Residues191Help icon, hover over for help text.
Mean Residue Weight117.2Help icon, hover over for help text.
Data Processing Software NameCDToolHelp icon, hover over for help text.
Data Processing Software Version1.4Help icon, hover over for help text.
Number of Smoothing Points7Help icon, hover over for help text.
Wavelength Range for Zeroing263-270Help icon, hover over for help text.

Sec. Struct.

PCDDBIDCD0004676000Help icon, hover over for help text.
Secondary Structure Calculated fromcrystal structureHelp icon, hover over for help text.
DSSP value: alpha helix0.584Help icon, hover over for help text.
DSSP value: 3-10 helix0.037Help icon, hover over for help text.
DSSP value: pi helix0.000Help icon, hover over for help text.
DSSP value: beta strand0.021Help icon, hover over for help text.
DSSP value: beta bridge0.010Help icon, hover over for help text.
DSSP value: bonded turn0.118Help icon, hover over for help text.
DSSP value: bend0.047Help icon, hover over for help text.
DSSP value: loop or irregular0.183Help icon, hover over for help text.

Info.

PCDDBIDCD0004676000Help icon, hover over for help text.
PDB ID2CCM: RSCBpdb PDBj PDBeHelp icon, hover over for help text.
UniProt IDO76764 Help icon, hover over for help text.
Enzyme Classification (EC)No data providedHelp icon, hover over for help text.
Medline EntryNo data providedHelp icon, hover over for help text.
Cath ClassificationNo data providedHelp icon, hover over for help text.
SequenceMAAHQLSDFQ RNKILRVFNT FYDCNHDGVI EWDDFELAIK KICNLHSWPT DGKKHNEARA TLKLIWDGLR KYADENEDEQ VTKEEWLKMW AECVKSVEKG ESLPEWLTKY MNFMFDVNDT SGDNIIDKHE YSTVYMSYGI PKSDCDAAFD TLSDGGKTMV TREIFARLWT EYFVSNDRGA KGNHLFGTLK L Help icon, hover over for help text.
Type of proteinsoluble globularHelp icon, hover over for help text.
Keyword/phrase Acalcium sensorHelp icon, hover over for help text.
Keyword/phrase BNo data providedHelp icon, hover over for help text.
Keyword/phrase CNo data providedHelp icon, hover over for help text.
Keyword/phrase DNo data providedHelp icon, hover over for help text.
Keyword/phrase ENo data providedHelp icon, hover over for help text.
Keyword/phrase FNo data providedHelp icon, hover over for help text.
Keyword/phrase GNo data providedHelp icon, hover over for help text.
Keyword/phrase HNo data providedHelp icon, hover over for help text.
Keyword/phrase INo data providedHelp icon, hover over for help text.
Keyword/phrase JNo data providedHelp icon, hover over for help text.
Publication AuthorsP. T. Erskine, A. Fokas, C. Muriithi, H. Rehman, L. A. Yates, A. Bowyer,I. S. Findlow, R. Hagan, J. M. Werner, A. J. Miles, B. A. Wallace S. A. Wells, S. P. Wood and J. B. CooperHelp icon, hover over for help text.
Publication Year2015Help icon, hover over for help text.
Publication JournalActa Cryst DHelp icon, hover over for help text.
Publication Title X-ray, spectroscopic and normal-mode dynamics of calexcitin: structure-function studies of a neuronal calcium-signalling proteinHelp icon, hover over for help text.
Publication Volume71Help icon, hover over for help text.
Publication Pages615-631Help icon, hover over for help text.
Citation PublicationNo data providedHelp icon, hover over for help text.

Depositor

PCDDBIDCD0004676000Help icon, hover over for help text.
Depositor NameAndrew MilesHelp icon, hover over for help text.
Department/School namecrystallographyHelp icon, hover over for help text.
University/Institution/CorporationBirkbeckHelp icon, hover over for help text.
Depositor CountryUnited KingdomHelp icon, hover over for help text.
Name of Principal Investigator (if not depositor)B. A. WallaceHelp icon, hover over for help text.

Validation

PCDDBID CD0004676000 Help icon, hover over for help text.
Missing Wavelengths PASS Help icon, hover over for help text.
Maximum Delta Epsilon PASS Help icon, hover over for help text.
Minimal Level of Maximum Delta Epsilon PASS Help icon, hover over for help text.
Peak Locations PASS Help icon, hover over for help text.
Feature Width PASS Help icon, hover over for help text.
Experimental Temperature PASS Help icon, hover over for help text.
UniProt sequence PASS Help icon, hover over for help text.
Molecular Weight PASS Help icon, hover over for help text.
Number of Residues PASS Help icon, hover over for help text.
Mean Residue Weight value PASS Help icon, hover over for help text.
Concentration and Pathlength PASS Help icon, hover over for help text.
CSA / ACS peak ratio PASS Help icon, hover over for help text.
CSA / ACS Temperature PASS Help icon, hover over for help text.
Peak Shift test PASS Help icon, hover over for help text.
Standard Deviation PASS Help icon, hover over for help text.
Noise: 260-270nm PASS Help icon, hover over for help text.
Flat topped peaks PASS Help icon, hover over for help text.
Wavelength range PASS Help icon, hover over for help text.
Interval resolution PASS Help icon, hover over for help text.
High Tension Voltage 240-260nm PASS Help icon, hover over for help text.
Projection Test PASS Help icon, hover over for help text.
Standard Deviation At Peak PASS Help icon, hover over for help text.
Overall validation level is: PASS
Validation report compiled by Validichro v1.2.5, 2015-03-14, 5:39pm.
Depositor comment for validation report :
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