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Record for CD0000115000 (Large-conductance mechanosensitive channel)

Download the PCDDB data file for this record

Sample

PCDDBIDCD0000115000
Protein NameLarge-conductance mechanosensitive channel
Alternative Protein NamesMscL
Source OrganismMycobacterium tuberculosis
Protein SupplierR.H.Spencer, California Institute of Technology
Expression System or natural sourceE. coli
Expressed AsWild-type
Mutation DetailsNo data provided
Expression tags (if any)No data provided
Ligands Present and Concentration or ratioNo data provided
Macromolecular Partner(s) and Concentration or ratioNo data provided
Deposition Date2012-01-31

Spectra

PCDDBIDCD0000115000
Raw Sample Spectra file   chart
Dichroism Units of Raw Sample DataDRS units
HT / High Voltage / Dynode Spectra file   chart
Raw Baseline Spectra file   chart
Dichroism Units of Raw Baseline DataDRS units
Average Sample file   chart
Dichroism Units of Average Sample DataDRS units
Averaged Baseline file   chart
Dichroism Units of Averaged Baseline DataDRS units
Net Smoothed Spectrum file   chart
Dichroism Units of Net Smoothed DataDRS units
Final Processed Spectrum file   chart
Dichroism Units of Processed DataDelta Epsilon

Experiment

PCDDBIDCD0000115000
CD or SRCDSRCD
Protein Concentration (mg/ml)4.87
Concentration Quantification MethodExtinction Coefficient
Protein Purity (%)No data provided
Purity Quantification MethodNo data provided
Buffer Contents and Concentrations20mM Tris pH 7.5, 20mM NaCl, 0.05% DDM
Baseline Contents20mM Tris pH 7.5, 20mM NaCl, 0.05% DDM
Experimental Temperature (C)10
Instrument or beamlineDaresbury 3.1
Detector Angle (Scattering Angle)No data provided
Sample Cell Pathlength (cm)0.0013
Cell Pathlength Calibration MethodInterferometry
Sample Cell TypeCylindrical-Demountable
Sample Cell CompositionSuprasil
Sample Chamber AtmosphereNitrogen
Number of repeat scans4
Continuous or Stepped scanStepped
Maximum (highest) wavelength, nm280
Minimum (lowest) wavelength, nm175
Criteria for low wavelength cutoffNoise level
Wavelength interval, nm1
Dwell or Averaging time, seconds3
Experimental Collection date2003-05-02
Local Spectrum Identifiert16838

Calibration

PCDDBIDCD0000115000
CSA or ACSCSA
CSA/ACS Standard Spectrum file   chart
Dichroism Units for CSA StandardDRS units
Final Spectrum CalibratedYES
CSA/ACS Standard Concentration (mg/ml)9.33
CSA/ACS Pathlength (mm)0.1
CSA/ACS Zeroed at-
CSA/ACS Date Measured2003-05-02
CSA/ACS Ratio (192.5nm and 290.0 nm)1.98
CD signal at 290nm (mdeg)317329
CSA/ACS Experiment temperature, C25

Data Process

PCDDBIDCD0000115000
Molecular Weight16023.4
Number of Residues151
Mean Residue Weight106.8
Data Processing Software NameCDTool
Data Processing Software Version1.4
Number of Smoothing Points7
Wavelength Range for Zeroing263-267

Sec. Struct.

PCDDBIDCD0000115000
Secondary Structure Calculated fromCrystal structure
DSSP value: alpha helix0.528
DSSP value: 3-10 helix0.000
DSSP value: pi helix0.000
DSSP value: beta strand0.034
DSSP value: beta bridge0.000
DSSP value: bonded turn0.036
DSSP value: bend0.013
DSSP value: loop or irregular0.390

Info.

PCDDBIDCD0000115000
PDB ID2oar: RSCBpdb PDBj PDBe
UniProt IDA5U127
Enzyme Classification (EC)-
Medline Entry21505036 (pubmed)
Cath Classification1.20.5.220
SequenceMLKGFKEFLA RGNIVDLAVA VVIGTAFTAL VTKFTDSIIT PLINRIGVNA QSDVGILRIG IGGGQTIDLN VLLSAAINFF LIAFAVYFLV VLPYNTLRKK GEVEQPGDTQ VVLLTEIRDL LAQTNGDSPG RHGGRGTPSP TDGPRASTES Q
Type of proteinmembrane
Keyword/phrase AMP180
Keyword/phrase BSMP180
Keyword/phrase Cmembrane protein
Keyword/phrase DNo data provided
Keyword/phrase ENo data provided
Keyword/phrase FNo data provided
Keyword/phrase GNo data provided
Keyword/phrase HNo data provided
Keyword/phrase INo data provided
Keyword/phrase JNo data provided
Publication AuthorsAli Abdul-Gader, Andrew John Miles and B.A. Wallace
Publication Year2011
Publication JournalBioinformatics
Publication TitleA Reference Dataset for the Analyses of Membrane Protein Sec-ondary Structures and Transmembrane Residues using Circular Dichroism Spectroscopy
Publication Volume27
Publication Pages1630-1636
Citation Publication file

Depositor

PCDDBIDCD0000115000
Depositor NameAndrew Miles
Department/School namecrystallography
University/Institution/CorporationBirkbeck
Depositor CountryUnited Kingdom
Name of Principal Investigator (if not depositor)BA Wallace

Validation

PCDDBID CD0000115000
Missing Wavelengths PASS
Maximum Delta Epsilon PASS
Minimal Level of Maximum Delta Epsilon PASS
Peak Locations PASS
Feature Width PASS
Experimental Temperature PASS
UniProt sequence PASS
Molecular Weight PASS
Number of Residues PASS
Mean Residue Weight value PASS
Concentration and Pathlength PASS
CSA / ACS peak ratio PASS
CSA / ACS Temperature PASS
Peak Shift test PASS
Standard Deviation PASS
Noise: 260-270nm PASS
Flat topped peaks PASS
Wavelength range PASS
Interval resolution PASS
High Tension Voltage 240-260nm PASS
Projection Test PASS
Standard Deviation At Peak PASS
Overall validation level is: PASS
Validation report compiled by Validichro v1.2.5, 2012-01-31, 11:51am.
Depositor comment for validation report :
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