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Record for CD0000071000 (Ubiquitin)

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Sample

PCDDBIDCD0000071000Help icon, hover over for help text.
Protein NameUbiquitinHelp icon, hover over for help text.
Alternative Protein NamesUBCHelp icon, hover over for help text.
Source OrganismHomo sapiensHelp icon, hover over for help text.
Protein SupplierNo data providedHelp icon, hover over for help text.
Expression System or natural sourceE. coliHelp icon, hover over for help text.
Expressed AsWild-typeHelp icon, hover over for help text.
Mutation DetailsNo data providedHelp icon, hover over for help text.
Expression tags (if any)No data providedHelp icon, hover over for help text.
Ligands Present and Concentration or ratioNo data providedHelp icon, hover over for help text.
Macromolecular Partner(s) and Concentration or ratioNo data providedHelp icon, hover over for help text.
Deposition Date2009-11-28Help icon, hover over for help text.

Spectra

PCDDBIDCD0000071000Help icon, hover over for help text.
Raw Sample Spectra file   chartHelp icon, hover over for help text.
Dichroism Units of Raw Sample DataMillidegreesHelp icon, hover over for help text.
HT / High Voltage / Dynode Spectra file   chartHelp icon, hover over for help text.
Raw Baseline Spectra file   chartHelp icon, hover over for help text.
Dichroism Units of Raw Baseline DataMillidegreesHelp icon, hover over for help text.
Average Sample file   chartHelp icon, hover over for help text.
Dichroism Units of Average Sample DataMillidegreesHelp icon, hover over for help text.
Averaged Baseline file   chartHelp icon, hover over for help text.
Dichroism Units of Averaged Baseline DataMillidegreesHelp icon, hover over for help text.
Net Smoothed Spectrum file   chartHelp icon, hover over for help text.
Dichroism Units of Net Smoothed DataMillidegreesHelp icon, hover over for help text.
Final Processed Spectrum file   chartHelp icon, hover over for help text.
Dichroism Units of Processed DataDelta EpsilonHelp icon, hover over for help text.

Experiment

PCDDBIDCD0000071000Help icon, hover over for help text.
CD or SRCDSRCDHelp icon, hover over for help text.
Protein Concentration (mg/ml)6.8Help icon, hover over for help text.
Concentration Quantification MethodQAAHelp icon, hover over for help text.
Protein Purity (%)95Help icon, hover over for help text.
Purity Quantification MethodNo data providedHelp icon, hover over for help text.
Buffer Contents and ConcentrationswaterHelp icon, hover over for help text.
Baseline ContentswaterHelp icon, hover over for help text.
Experimental Temperature (C)4Help icon, hover over for help text.
Instrument or beamlineDaresbury 12.1Help icon, hover over for help text.
Detector Angle (Scattering Angle)No data providedHelp icon, hover over for help text.
Sample Cell Pathlength (cm)0.001Help icon, hover over for help text.
Cell Pathlength Calibration MethodInterferometryHelp icon, hover over for help text.
Sample Cell TypeCylindrical-DemountableHelp icon, hover over for help text.
Sample Cell CompositionCaF2Help icon, hover over for help text.
Sample Chamber AtmosphereNitrogenHelp icon, hover over for help text.
Number of repeat scans3Help icon, hover over for help text.
Continuous or Stepped scanNo data providedHelp icon, hover over for help text.
Maximum (highest) wavelength, nm279Help icon, hover over for help text.
Minimum (lowest) wavelength, nm175Help icon, hover over for help text.
Criteria for low wavelength cutoffHT valueHelp icon, hover over for help text.
Wavelength interval, nm1Help icon, hover over for help text.
Dwell or Averaging time, seconds1Help icon, hover over for help text.
Experimental Collection date2004-11-24Help icon, hover over for help text.
Local Spectrum Identifierr12676Help icon, hover over for help text.

Calibration

PCDDBIDCD0000071000Help icon, hover over for help text.
CSA or ACSCSAHelp icon, hover over for help text.
CSA/ACS Standard Spectrum file   chartHelp icon, hover over for help text.
Dichroism Units for CSA StandardMillidegrees (theta)Help icon, hover over for help text.
Final Spectrum CalibratedNo data providedHelp icon, hover over for help text.
CSA/ACS Standard Concentration (mg/ml)11.4Help icon, hover over for help text.
CSA/ACS Pathlength (mm)0.1Help icon, hover over for help text.
CSA/ACS Zeroed atNo data providedHelp icon, hover over for help text.
CSA/ACS Date Measured2004-11-24Help icon, hover over for help text.
CSA/ACS Ratio (192.5nm and 290.0 nm)1.96Help icon, hover over for help text.
CD signal at 290nm (mdeg)37.4Help icon, hover over for help text.
CSA/ACS Experiment temperature, C25Help icon, hover over for help text.

Data Process

PCDDBIDCD0000071000Help icon, hover over for help text.
Molecular Weight8565Help icon, hover over for help text.
Number of Residues76Help icon, hover over for help text.
Mean Residue Weight114.2Help icon, hover over for help text.
Data Processing Software NameCDToolHelp icon, hover over for help text.
Data Processing Software Version1.4Help icon, hover over for help text.
Number of Smoothing Points5Help icon, hover over for help text.
Wavelength Range for Zeroing263-270Help icon, hover over for help text.

Sec. Struct.

PCDDBIDCD0000071000Help icon, hover over for help text.
Secondary Structure Calculated fromPDB FileHelp icon, hover over for help text.
DSSP value: alpha helix0.158Help icon, hover over for help text.
DSSP value: 3-10 helix0.092Help icon, hover over for help text.
DSSP value: pi helix0.000Help icon, hover over for help text.
DSSP value: beta strand0.316Help icon, hover over for help text.
DSSP value: beta bridge0.026Help icon, hover over for help text.
DSSP value: bonded turn0.145Help icon, hover over for help text.
DSSP value: bend0.039Help icon, hover over for help text.
DSSP value: loop or irregular0.224Help icon, hover over for help text.

Info.

PCDDBIDCD0000071000Help icon, hover over for help text.
PDB ID1ubi: RSCBpdb PDBj PDBeHelp icon, hover over for help text.
UniProt IDP62988 Help icon, hover over for help text.
Enzyme Classification (EC)No data providedHelp icon, hover over for help text.
Medline Entry16787970 (pubmed)Help icon, hover over for help text.
Cath Classification3.10.20.90 Help icon, hover over for help text.
SequenceMQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGG Help icon, hover over for help text.
Type of proteinsoluble globularHelp icon, hover over for help text.
Keyword/phrase ASP175Help icon, hover over for help text.
Keyword/phrase Bsingle domain typeHelp icon, hover over for help text.
Keyword/phrase Cmixed alpha betaHelp icon, hover over for help text.
Keyword/phrase DNo data providedHelp icon, hover over for help text.
Keyword/phrase ENo data providedHelp icon, hover over for help text.
Keyword/phrase FNo data providedHelp icon, hover over for help text.
Keyword/phrase GNo data providedHelp icon, hover over for help text.
Keyword/phrase HNo data providedHelp icon, hover over for help text.
Keyword/phrase INo data providedHelp icon, hover over for help text.
Keyword/phrase JNo data providedHelp icon, hover over for help text.
Publication AuthorsLees, J.G., Wien, F., Miles, A.J. & Wallace, B.A.,Help icon, hover over for help text.
Publication Year2006Help icon, hover over for help text.
Publication JournalBioinformaticsHelp icon, hover over for help text.
Publication TitleA reference database for circular dichroism spectroscopy covering fold and secondary structure spaceHelp icon, hover over for help text.
Publication Volume22Help icon, hover over for help text.
Publication Pages1955-1962Help icon, hover over for help text.
Citation Publication fileHelp icon, hover over for help text.

Depositor

PCDDBIDCD0000071000Help icon, hover over for help text.
Depositor NameAndrew MilesHelp icon, hover over for help text.
Department/School nameCrystallographyHelp icon, hover over for help text.
University/Institution/CorporationBirkbeckHelp icon, hover over for help text.
Depositor CountryUnited KingdomHelp icon, hover over for help text.
Name of Principal Investigator (if not depositor)B.A. WallaceHelp icon, hover over for help text.

Validation

PCDDBID CD0000071000 Help icon, hover over for help text.
Maximum Delta Epsilon PASS Help icon, hover over for help text.
Minimal Level of Maximum Delta Epsilon PASS Help icon, hover over for help text.
Signal to noise ratio PASS Help icon, hover over for help text.
Deviations from zero PASS Help icon, hover over for help text.
High Tension Voltage readings PASS Help icon, hover over for help text.
Wavelength range covered (low end / high end) PASS PASS Help icon, hover over for help text.
Interval resolution PASS Help icon, hover over for help text.
Deviation between 260-270nm PASS Help icon, hover over for help text.
Mean Residue Weight value PASS Help icon, hover over for help text.
Flat topped peaks PASS Help icon, hover over for help text.
CSA or ACS standard used PASS Help icon, hover over for help text.
Width of peaks at half height PASS Help icon, hover over for help text.
Peak and trough locations PASS Help icon, hover over for help text.
Spectral Feature Width PASS Help icon, hover over for help text.
CSA / ACS peak ratio PASS Help icon, hover over for help text.
Calibration data at 290nm PASS Help icon, hover over for help text.
Sum of DSSP fractions PASS Help icon, hover over for help text.
Overall validation level is: PASS
Validation report compiled by Validichro v1.0, 2009-12-15, 13:00.
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