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Record for CD0000010000 (Beta galactosidase)

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Sample

PCDDBIDCD0000010000Help icon, hover over for help text.
Protein NameBeta galactosidaseHelp icon, hover over for help text.
Alternative Protein NameslactaseHelp icon, hover over for help text.
Source OrganismE.coliHelp icon, hover over for help text.
Protein SupplierNo data providedHelp icon, hover over for help text.
Expression System or natural sourceE. coliHelp icon, hover over for help text.
Expressed AsWild-typeHelp icon, hover over for help text.
Mutation DetailsNo data providedHelp icon, hover over for help text.
Expression tags (if any)No data providedHelp icon, hover over for help text.
Ligands Present and Concentration or ratioNo data providedHelp icon, hover over for help text.
Macromolecular Partner(s) and Concentration or ratioNo data providedHelp icon, hover over for help text.
Deposition Date2009-11-25Help icon, hover over for help text.

Spectra

PCDDBIDCD0000010000Help icon, hover over for help text.
Raw Sample Spectra file   chartHelp icon, hover over for help text.
Dichroism Units of Raw Sample DataMillidegreesHelp icon, hover over for help text.
HT / High Voltage / Dynode Spectra file   chartHelp icon, hover over for help text.
Raw Baseline Spectra file   chartHelp icon, hover over for help text.
Dichroism Units of Raw Baseline DataMillidegreesHelp icon, hover over for help text.
Average Sample file   chartHelp icon, hover over for help text.
Dichroism Units of Average Sample DataMillidegreesHelp icon, hover over for help text.
Averaged Baseline file   chartHelp icon, hover over for help text.
Dichroism Units of Averaged Baseline DataMillidegreesHelp icon, hover over for help text.
Net Smoothed Spectrum file   chartHelp icon, hover over for help text.
Dichroism Units of Net Smoothed DataMillidegreesHelp icon, hover over for help text.
Final Processed Spectrum file   chartHelp icon, hover over for help text.
Dichroism Units of Processed DataDelta EpsilonHelp icon, hover over for help text.

Experiment

PCDDBIDCD0000010000Help icon, hover over for help text.
CD or SRCDSRCDHelp icon, hover over for help text.
Protein Concentration (mg/ml)6.54Help icon, hover over for help text.
Concentration Quantification MethodQAAHelp icon, hover over for help text.
Protein Purity (%)95Help icon, hover over for help text.
Purity Quantification MethodNo data providedHelp icon, hover over for help text.
Buffer Contents and ConcentrationswaterHelp icon, hover over for help text.
Baseline ContentswaterHelp icon, hover over for help text.
Experimental Temperature (C)4Help icon, hover over for help text.
Instrument or beamlineISA UV1Help icon, hover over for help text.
Detector Angle (Scattering Angle)No data providedHelp icon, hover over for help text.
Sample Cell Pathlength (cm)0.0015Help icon, hover over for help text.
Cell Pathlength Calibration MethodInterferometryHelp icon, hover over for help text.
Sample Cell TypeCylindrical-DemountableHelp icon, hover over for help text.
Sample Cell CompositionSuprasilHelp icon, hover over for help text.
Sample Chamber AtmosphereNitrogenHelp icon, hover over for help text.
Number of repeat scans3Help icon, hover over for help text.
Continuous or Stepped scanNo data providedHelp icon, hover over for help text.
Maximum (highest) wavelength, nm300Help icon, hover over for help text.
Minimum (lowest) wavelength, nm175Help icon, hover over for help text.
Criteria for low wavelength cutoffHT valueHelp icon, hover over for help text.
Wavelength interval, nm1Help icon, hover over for help text.
Dwell or Averaging time, seconds3Help icon, hover over for help text.
Experimental Collection date2003-04-30Help icon, hover over for help text.
Local Spectrum Identifiera2516Help icon, hover over for help text.

Calibration

PCDDBIDCD0000010000Help icon, hover over for help text.
CSA or ACSNo data providedHelp icon, hover over for help text.
CSA/ACS Standard Spectrum file   chartHelp icon, hover over for help text.
Dichroism Units for CSA StandardMillidegrees (theta)Help icon, hover over for help text.
Final Spectrum CalibratedNo data providedHelp icon, hover over for help text.
CSA/ACS Standard Concentration (mg/ml)1.04Help icon, hover over for help text.
CSA/ACS Pathlength (mm)1Help icon, hover over for help text.
CSA/ACS Zeroed at235-245Help icon, hover over for help text.
CSA/ACS Date Measured2003-04-30Help icon, hover over for help text.
CSA/ACS Ratio (192.5nm and 290.0 nm)1.89Help icon, hover over for help text.
CD signal at 290nm (mdeg)29.76Help icon, hover over for help text.
CSA/ACS Experiment temperature, C25Help icon, hover over for help text.

Data Process

PCDDBIDCD0000010000Help icon, hover over for help text.
Molecular Weight116353Help icon, hover over for help text.
Number of Residues1023Help icon, hover over for help text.
Mean Residue Weight113.8Help icon, hover over for help text.
Data Processing Software NameCDToolHelp icon, hover over for help text.
Data Processing Software Version1.4Help icon, hover over for help text.
Number of Smoothing Points5Help icon, hover over for help text.
Wavelength Range for Zeroing263-267Help icon, hover over for help text.

Sec. Struct.

PCDDBIDCD0000010000Help icon, hover over for help text.
Secondary Structure Calculated fromPDB FileHelp icon, hover over for help text.
DSSP value: alpha helix0.103Help icon, hover over for help text.
DSSP value: 3-10 helix0.036Help icon, hover over for help text.
DSSP value: pi helix0.000Help icon, hover over for help text.
DSSP value: beta strand0.364Help icon, hover over for help text.
DSSP value: beta bridge0.025Help icon, hover over for help text.
DSSP value: bonded turn0.113Help icon, hover over for help text.
DSSP value: bend0.114Help icon, hover over for help text.
DSSP value: loop or irregular0.245Help icon, hover over for help text.

Info.

PCDDBIDCD0000010000Help icon, hover over for help text.
PDB ID1bgl: RSCBpdb PDBj PDBeHelp icon, hover over for help text.
UniProt IDP00722 Help icon, hover over for help text.
Enzyme Classification (EC)3.2.1.23Help icon, hover over for help text.
Medline Entry16787970 (pubmed)Help icon, hover over for help text.
Cath Classification2.60.120.260 2.60.40.320 3.20.20.80 2.60.40.320 2.70.98.10 Help icon, hover over for help text.
SequenceTMITDSLAVV LQRRDWENPG VTQLNRLAAH PPFASWRNSE EARTDRPSQQ LRSLNGEWRF AWFPAPEAVP ESWLECDLPE ADTVVVPSNW QMHGYDAPIY TNVTYPITVN PPFVPTENPT GCYSLTFNVD ESWLQEGQTR IIFDGVNSAF HLWCNGRWVG YGQDSRLPSE FDLSAFLRAG ENRLAVMVLR WSDGSYLEDQ DMWRMSGIFR DVSLLHKPTT QISDFHVATR FNDDFSRAVL EAEVQMCGEL RDYLRVTVSL WQGETQVASG TAPFGGEIID ERGGYADRVT LRLNVENPKL WSAEIPNLYR AVVELHTADG TLIEAEACDV GFREVRIENG LLLLNGKPLL IRGVNRHEHH PLHGQVMDEQ TMVQDILLMK QNNFNAVRCS HYPNHPLWYT LCDRYGLYVV DEANIETHGM VPMNRLTDDP RWLPAMSERV TRMVQRDRNH PSVIIWSLGN ESGHGANHDA LYRWIKSVDP SRPVQYEGGG ADTTATDIIC PMYARVDEDQ PFPAVPKWSI KKWLSLPGET RPLILCEYAH AMGNSLGGFA KYWQAFRQYP RLQGGFVWDW VDQSLIKYDE NGNPWSAYGG DFGDTPNDRQ FCMNGLVFAD RTPHPALTEA KHQQQFFQFR LSGQTIEVTS EYLFRHSDNE LLHWMVALDG KPLASGEVPL DVAPQGKQLI ELPELPQPES AGQLWLTVRV VQPNATAWSE AGHISAWQQW RLAENLSVTL PAASHAIPHL TTSEMDFCIE LGNKRWQFNR QSGFLSQMWI GDKKQLLTPL RDQFTRAPLD NDIGVSEATR IDPNAWVERW KAAGHYQAEA ALLQCTADTL ADAVLITTAH AWQHQGKTLF ISRKTYRIDG SGQMAITVDV EVASDTPHPA RIGLNCQLAQ VAERVNWLGL GPQENYPDRL TAACFDRWDL PLSDMYTPYV FPSENGLRCG TRELNYGPHQ WRGDFQFNIS RYSQQQLMET SHRHLLHAEE GTWLNIDGFH MGIGGDDSWS PSVSAEFQLS AGRYHYQLVW CQK Help icon, hover over for help text.
Type of proteinsoluble globularHelp icon, hover over for help text.
Keyword/phrase ASP175Help icon, hover over for help text.
Keyword/phrase Bmulti domain typeHelp icon, hover over for help text.
Keyword/phrase CbetaHelp icon, hover over for help text.
Keyword/phrase DNo data providedHelp icon, hover over for help text.
Keyword/phrase ENo data providedHelp icon, hover over for help text.
Keyword/phrase FNo data providedHelp icon, hover over for help text.
Keyword/phrase GNo data providedHelp icon, hover over for help text.
Keyword/phrase HNo data providedHelp icon, hover over for help text.
Keyword/phrase INo data providedHelp icon, hover over for help text.
Keyword/phrase JNo data providedHelp icon, hover over for help text.
Publication AuthorsLees, J.G., Wien, F., Miles, A.J. & Wallace, B.A.,Help icon, hover over for help text.
Publication Year2006Help icon, hover over for help text.
Publication JournalBioinformaticsHelp icon, hover over for help text.
Publication TitleA reference database for circular dichroism spectroscopy covering fold and secondary structure spaceHelp icon, hover over for help text.
Publication Volume22Help icon, hover over for help text.
Publication Pages1955-1962Help icon, hover over for help text.
Citation Publication fileHelp icon, hover over for help text.

Depositor

PCDDBIDCD0000010000Help icon, hover over for help text.
Depositor NameAndrew MilesHelp icon, hover over for help text.
Department/School nameCrystallographyHelp icon, hover over for help text.
University/Institution/CorporationBirkbeckHelp icon, hover over for help text.
Depositor CountryUnited KingdomHelp icon, hover over for help text.
Name of Principal Investigator (if not depositor)B.A. WallaceHelp icon, hover over for help text.

Validation

PCDDBID CD0000010000 Help icon, hover over for help text.
Maximum Delta Epsilon PASS Help icon, hover over for help text.
Minimal Level of Maximum Delta Epsilon PASS Help icon, hover over for help text.
Signal to noise ratio PASS Help icon, hover over for help text.
Deviations from zero PASS Help icon, hover over for help text.
High Tension Voltage readings PASS Help icon, hover over for help text.
Wavelength range covered (low end / high end) PASS PASS Help icon, hover over for help text.
Interval resolution PASS Help icon, hover over for help text.
Deviation between 260-270nm PASS Help icon, hover over for help text.
Mean Residue Weight value PASS Help icon, hover over for help text.
Flat topped peaks PASS Help icon, hover over for help text.
CSA or ACS standard used PASS Help icon, hover over for help text.
Width of peaks at half height PASS Help icon, hover over for help text.
Peak and trough locations PASS Help icon, hover over for help text.
Spectral Feature Width PASS Help icon, hover over for help text.
CSA / ACS peak ratio PASS Help icon, hover over for help text.
Calibration data at 290nm PASS Help icon, hover over for help text.
Sum of DSSP fractions PASS Help icon, hover over for help text.
Overall validation level is: PASS
Validation report compiled by Validichro v1.0, 2009-12-15, 13:00.
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