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Record for CD0000010000 (Beta galactosidase)

Download the PCDDB data file for this record

Sample

PCDDBIDCD0000010000
Protein NameBeta galactosidase
Alternative Protein Nameslactase
Source OrganismE.coli
Protein SupplierNo data provided
Expression System or natural sourceE. coli
Expressed AsWild-type
Mutation DetailsNo data provided
Expression tags (if any)No data provided
Ligands Present and Concentration or ratioNo data provided
Macromolecular Partner(s) and Concentration or ratioNo data provided
Deposition Date2009-11-25

Spectra

PCDDBIDCD0000010000
Raw Sample Spectra file   chart
Dichroism Units of Raw Sample DataMillidegrees
HT / High Voltage / Dynode Spectra file   chart
Raw Baseline Spectra file   chart
Dichroism Units of Raw Baseline DataMillidegrees
Average Sample file   chart
Dichroism Units of Average Sample DataMillidegrees
Averaged Baseline file   chart
Dichroism Units of Averaged Baseline DataMillidegrees
Net Smoothed Spectrum file   chart
Dichroism Units of Net Smoothed DataMillidegrees
Final Processed Spectrum file   chart
Dichroism Units of Processed DataDelta Epsilon

Experiment

PCDDBIDCD0000010000
CD or SRCDSRCD
Protein Concentration (mg/ml)6.54
Concentration Quantification MethodQAA
Protein Purity (%)95
Purity Quantification MethodNo data provided
Buffer Contents and Concentrationswater
Baseline Contentswater
Experimental Temperature (C)4
Instrument or beamlineISA UV1
Detector Angle (Scattering Angle)No data provided
Sample Cell Pathlength (cm)0.0015
Cell Pathlength Calibration MethodInterferometry
Sample Cell TypeCylindrical-Demountable
Sample Cell CompositionSuprasil
Sample Chamber AtmosphereNitrogen
Number of repeat scans3
Continuous or Stepped scanNo data provided
Maximum (highest) wavelength, nm300
Minimum (lowest) wavelength, nm175
Criteria for low wavelength cutoffHT value
Wavelength interval, nm1
Dwell or Averaging time, seconds3
Experimental Collection date2003-04-30
Local Spectrum Identifiera2516

Calibration

PCDDBIDCD0000010000
CSA or ACSNo data provided
CSA/ACS Standard Spectrum file   chart
Dichroism Units for CSA StandardMillidegrees (theta)
Final Spectrum CalibratedNo data provided
CSA/ACS Standard Concentration (mg/ml)1.04
CSA/ACS Pathlength (mm)1
CSA/ACS Zeroed at235-245
CSA/ACS Date Measured2003-04-30
CSA/ACS Ratio (192.5nm and 290.0 nm)1.89
CD signal at 290nm (mdeg)29.76
CSA/ACS Experiment temperature, C25

Data Process

PCDDBIDCD0000010000
Molecular Weight116353
Number of Residues1023
Mean Residue Weight113.8
Data Processing Software NameCDTool
Data Processing Software Version1.4
Number of Smoothing Points5
Wavelength Range for Zeroing263-267

Sec. Struct.

PCDDBIDCD0000010000
Secondary Structure Calculated fromPDB File
DSSP value: alpha helix0.103
DSSP value: 3-10 helix0.036
DSSP value: pi helix0.000
DSSP value: beta strand0.364
DSSP value: beta bridge0.025
DSSP value: bonded turn0.113
DSSP value: bend0.114
DSSP value: loop or irregular0.245

Info.

PCDDBIDCD0000010000
PDB ID1bgl: RSCBpdb PDBj PDBe
UniProt IDP00722
Enzyme Classification (EC)3.2.1.23
Medline Entry16787970 (pubmed)
Cath Classification2.60.120.260 2.60.40.320 3.20.20.80 2.60.40.320 2.70.98.10
SequenceTMITDSLAVV LQRRDWENPG VTQLNRLAAH PPFASWRNSE EARTDRPSQQ LRSLNGEWRF AWFPAPEAVP ESWLECDLPE ADTVVVPSNW QMHGYDAPIY TNVTYPITVN PPFVPTENPT GCYSLTFNVD ESWLQEGQTR IIFDGVNSAF HLWCNGRWVG YGQDSRLPSE FDLSAFLRAG ENRLAVMVLR WSDGSYLEDQ DMWRMSGIFR DVSLLHKPTT QISDFHVATR FNDDFSRAVL EAEVQMCGEL RDYLRVTVSL WQGETQVASG TAPFGGEIID ERGGYADRVT LRLNVENPKL WSAEIPNLYR AVVELHTADG TLIEAEACDV GFREVRIENG LLLLNGKPLL IRGVNRHEHH PLHGQVMDEQ TMVQDILLMK QNNFNAVRCS HYPNHPLWYT LCDRYGLYVV DEANIETHGM VPMNRLTDDP RWLPAMSERV TRMVQRDRNH PSVIIWSLGN ESGHGANHDA LYRWIKSVDP SRPVQYEGGG ADTTATDIIC PMYARVDEDQ PFPAVPKWSI KKWLSLPGET RPLILCEYAH AMGNSLGGFA KYWQAFRQYP RLQGGFVWDW VDQSLIKYDE NGNPWSAYGG DFGDTPNDRQ FCMNGLVFAD RTPHPALTEA KHQQQFFQFR LSGQTIEVTS EYLFRHSDNE LLHWMVALDG KPLASGEVPL DVAPQGKQLI ELPELPQPES AGQLWLTVRV VQPNATAWSE AGHISAWQQW RLAENLSVTL PAASHAIPHL TTSEMDFCIE LGNKRWQFNR QSGFLSQMWI GDKKQLLTPL RDQFTRAPLD NDIGVSEATR IDPNAWVERW KAAGHYQAEA ALLQCTADTL ADAVLITTAH AWQHQGKTLF ISRKTYRIDG SGQMAITVDV EVASDTPHPA RIGLNCQLAQ VAERVNWLGL GPQENYPDRL TAACFDRWDL PLSDMYTPYV FPSENGLRCG TRELNYGPHQ WRGDFQFNIS RYSQQQLMET SHRHLLHAEE GTWLNIDGFH MGIGGDDSWS PSVSAEFQLS AGRYHYQLVW CQK
Type of proteinsoluble globular
Keyword/phrase ASP175
Keyword/phrase Bmulti domain type
Keyword/phrase Cbeta
Keyword/phrase DNo data provided
Keyword/phrase ENo data provided
Keyword/phrase FNo data provided
Keyword/phrase GNo data provided
Keyword/phrase HNo data provided
Keyword/phrase INo data provided
Keyword/phrase JNo data provided
Publication AuthorsLees, J.G., Wien, F., Miles, A.J. & Wallace, B.A.,
Publication Year2006
Publication JournalBioinformatics
Publication TitleA reference database for circular dichroism spectroscopy covering fold and secondary structure space
Publication Volume22
Publication Pages1955-1962
Citation Publication file

Depositor

PCDDBIDCD0000010000
Depositor NameAndrew Miles
Department/School nameCrystallography
University/Institution/CorporationBirkbeck
Depositor CountryUnited Kingdom
Name of Principal Investigator (if not depositor)B.A. Wallace

Validation

PCDDBID CD0000010000
Maximum Delta Epsilon PASS
Minimal Level of Maximum Delta Epsilon PASS
Signal to noise ratio PASS
Deviations from zero PASS
High Tension Voltage readings PASS
Wavelength range covered (low end / high end) PASS PASS
Interval resolution PASS
Deviation between 260-270nm PASS
Mean Residue Weight value PASS
Flat topped peaks PASS
CSA or ACS standard used PASS
Width of peaks at half height PASS
Peak and trough locations PASS
Spectral Feature Width PASS
CSA / ACS peak ratio PASS
Calibration data at 290nm PASS
Sum of DSSP fractions PASS
Overall validation level is: PASS
Validation report compiled by Validichro v1.0, 2009-12-15, 13:00.
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