PCDDB logo

Protein Circular Dichroism
Data Bank

Home | Search | Download | Deposit

Lost Password | Help
Create account
Login ID 
Password 

Record for CD0000005000 (Alpha chymotrypsin)

Download the PCDDB data file for this record

Sample

PCDDBIDCD0000005000
Protein NameAlpha chymotrypsin
Alternative Protein NamesNo data provided
Source OrganismBos taurus
Protein SupplierNo data provided
Expression System or natural sourcenatural source
Expressed AsWild-type
Mutation DetailsNo data provided
Expression tags (if any)No data provided
Ligands Present and Concentration or ratioNo data provided
Macromolecular Partner(s) and Concentration or ratioNo data provided
Deposition Date2009-11-25

Spectra

PCDDBIDCD0000005000
Raw Sample Spectra file   chart
Dichroism Units of Raw Sample DataDaresbury Arbitrary Units
HT / High Voltage / Dynode Spectra file   chart
Raw Baseline Spectra file   chart
Dichroism Units of Raw Baseline DataDaresbury Arbitrary Units
Average Sample file   chart
Dichroism Units of Average Sample DataDaresbury Arbitrary Units
Averaged Baseline file   chart
Dichroism Units of Averaged Baseline DataDaresbury Arbitrary Units
Net Smoothed Spectrum file   chart
Dichroism Units of Net Smoothed DataDaresbury Arbitrary Units
Final Processed Spectrum file   chart
Dichroism Units of Processed DataDelta Epsilon

Experiment

PCDDBIDCD0000005000
CD or SRCDSRCD
Protein Concentration (mg/ml)11.5
Concentration Quantification MethodQAA
Protein Purity (%)No data provided
Purity Quantification MethodNo data provided
Buffer Contents and Concentrationswater
Baseline Contentswater
Experimental Temperature (C)4
Instrument or beamlineDaresbury 12.1
Detector Angle (Scattering Angle)No data provided
Sample Cell Pathlength (cm)0.0015
Cell Pathlength Calibration MethodInterferometry
Sample Cell TypeCylindrical-Demountable
Sample Cell CompositionSuprasil
Sample Chamber AtmosphereNitrogen
Number of repeat scans3
Continuous or Stepped scanNo data provided
Maximum (highest) wavelength, nm280
Minimum (lowest) wavelength, nm175
Criteria for low wavelength cutoffHT value
Wavelength interval, nm1
Dwell or Averaging time, seconds1
Experimental Collection date2003-09-15
Local Spectrum Identifierr6218

Calibration

PCDDBIDCD0000005000
CSA or ACSNo data provided
CSA/ACS Standard Spectrum file   chart
Dichroism Units for CSA StandardDRS units
Final Spectrum CalibratedNo data provided
CSA/ACS Standard Concentration (mg/ml)10.3
CSA/ACS Pathlength (mm)0.1
CSA/ACS Zeroed at235-245
CSA/ACS Date Measured2003-09-15
CSA/ACS Ratio (192.5nm and 290.0 nm)2.02
CD signal at 290nm (mdeg)No data provided
CSA/ACS Experiment temperature, C25

Data Process

PCDDBIDCD0000005000
Molecular Weight25244
Number of Residues241
Mean Residue Weight105.2
Data Processing Software NameCDTool
Data Processing Software Version1.4
Number of Smoothing Points5
Wavelength Range for Zeroing263-267

Sec. Struct.

PCDDBIDCD0000005000
Secondary Structure Calculated fromPDB File
DSSP value: alpha helix0.091
DSSP value: 3-10 helix0.025
DSSP value: pi helix0.000
DSSP value: beta strand0.320
DSSP value: beta bridge0.027
DSSP value: bonded turn0.122
DSSP value: bend0.114
DSSP value: loop or irregular0.301

Info.

PCDDBIDCD0000005000
PDB ID5cha: RSCBpdb PDBj PDBe
UniProt IDP00766
Enzyme Classification (EC)3.4.21.1
Medline Entry16787970 (pubmed)
Cath Classification2.40.10.10
SequenceCGVPAIQPVL SGLIVNGEEA VPGSWPWQVS LQDKTGFHFC GGSLINENWV VTAAHCGVTT SDVVVAGEFD QGSSSEKIQK LKIAKVFKNS KYNSLTINND ITLLKLSTAA SFSQTVSAVC LPSASDDFAA GTTCVTTGWG LTRYANTPDR LQQASLPLLS NTNCKKYWGT KIKDAMICAG ASGVSSCMGD SGGPLVCKKN GAWTLVGIVS WGSSTCSTST PGVYARVTAL VNWVQQTLAA N
Type of proteinsoluble globular
Keyword/phrase ASP175
Keyword/phrase Bsingle domain type
Keyword/phrase Cbeta
Keyword/phrase DNo data provided
Keyword/phrase ENo data provided
Keyword/phrase FNo data provided
Keyword/phrase GNo data provided
Keyword/phrase HNo data provided
Keyword/phrase INo data provided
Keyword/phrase JNo data provided
Publication AuthorsLees, J.G., Wien, F., Miles, A.J. & Wallace, B.A.,
Publication Year2006
Publication JournalBioinformatics
Publication TitleA reference database for circular dichroism spectroscopy covering fold and secondary structure space
Publication Volume22
Publication Pages1955-1962
Citation Publication file

Depositor

PCDDBIDCD0000005000
Depositor NameAndrew Miles
Department/School nameCrystallography
University/Institution/CorporationBirkbeck
Depositor CountryUnited Kingdom
Name of Principal Investigator (if not depositor)B.A. Wallace

Validation

PCDDBID CD0000005000
Maximum Delta Epsilon PASS
Minimal Level of Maximum Delta Epsilon PASS
Signal to noise ratio PASS
Deviations from zero PASS
High Tension Voltage readings PASS
Wavelength range covered (low end / high end) PASS PASS
Interval resolution PASS
Deviation between 260-270nm PASS
Mean Residue Weight value PASS
Flat topped peaks PASS
CSA or ACS standard used PASS
Width of peaks at half height PASS
Peak and trough locations PASS
Spectral Feature Width PASS
CSA / ACS peak ratio PASS
Calibration data at 290nm N/A
Sum of DSSP fractions PASS
Overall validation level is: PASS
Validation report compiled by Validichro v1.0, 2009-12-15, 13:00.
Show data in a long page without tabs

Link to PCDDB Twitter account.
Link to PCDDB YouTube account.

The PCDDB is a development of the Department of Crystallography, Institute of Structural and Molecular Biology, Birkbeck College, University of London and the School of Biological and Chemical Sciences, Queen Mary University of London, UK. It is supported by a grant from the BBSRC. Copyright of the design and implementation of this site are retained by the Schools and the authors. © 2006-2017